9DRC

Ternary substrate complex of DNA polymerase iota R71A mutant with DNA (template A) and dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-16
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 65 2 2
Unit cell lengths	97.278, 97.278, 202.828
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.890 - 2.450
R-factor	0.2529
Rwork	0.248
R-free	0.29410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.480
High resolution limit [Å]	2.450	2.450
Rpim	0.025	0.443
Number of reflections	33634	665
<I/σ(I)>	24.1	1.5
Completeness [%]	100.0	100
Redundancy	8	5.7
CC(1/2)	0.999	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	100 mM HEPES pH 7 1.5 - 1.8 M Ammonium Sulfate 5 mM EDTA