9DRB

Binary complex of DNA polymerase iota with product DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-22
Detector	NOIR-1
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	97.662, 97.662, 202.376
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.980 - 2.360
R-factor	0.286
Rwork	0.284
R-free	0.32400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.980	2.460
High resolution limit [Å]	2.360	2.360
Rpim	0.019	0.315
Number of reflections	24479	2981
<I/σ(I)>	23	2.2
Completeness [%]	100.0	100
Redundancy	17.9	10
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	100 mM HEPES pH 7 1.5 - 1.8 M Ammonium Sulfate 5 mM EDTA