9DR0

Hare calicivirus protruding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	54.475, 82.972, 74.566
Unit cell angles	90.00, 90.89, 90.00

Refinement procedure

Resolution	44.310 - 1.550
R-factor	0.1617
Rwork	0.161
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.310	1.580
High resolution limit [Å]	1.550	1.550
Rmerge		1.335
Number of reflections	94671	9173
<I/σ(I)>	7.6
Completeness [%]	99.5
Redundancy	4
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M magnesium acetate tetrahydrate, 0.1 M MES, pH 6.5, 20% w/v PEG8000