9DQT

Binary substrate complex of DNA polymerase iota with DNA (template A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-04
Detector	NOIR-1
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	97.639, 97.639, 188.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.820 - 3.190
R-factor	0.3199
Rwork	0.317
R-free	0.36370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.820	3.510
High resolution limit [Å]	3.190	3.190
Rpim	0.042	0.424
Number of reflections	9431	2283
<I/σ(I)>	17.4	2.1
Completeness [%]	100.0	100
Redundancy	20.5	19.7
CC(1/2)	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	100 mM HEPES pH 7 1.5 -1.8 M Ammonium Sulfate 5 mM EDTA