9DQS

Structure of Tudor domain from Mycobacterium smegmatis UvrD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 2 21 21
Unit cell lengths	42.241, 53.390, 109.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.010 - 2.590
Rwork	0.234
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.390	2.700
High resolution limit [Å]	2.590	2.590
Rmerge	0.138
Number of reflections	8030	814
<I/σ(I)>	10.8
Completeness [%]	97.5	84.5
Redundancy	9.2	6
CC(1/2)	0.994	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	0.1 M Bis-Tris pH 5.5, 0.2 M NaCl, 25% PEG 3350