9DQK
human ClpP - Apo - A192E / E196R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95358 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.320, 94.990, 134.360 |
| Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
| Resolution | 49.070 - 2.750 |
| R-factor | 0.2073 |
| Rwork | 0.206 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.426 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.670 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Number of reflections | 179085 | 5951 |
| <I/σ(I)> | 10.6 | 0.67 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.990 | 0.190 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 200 mM potassium acetate, 35% (v/v) pentaerythritol propoxylate (5/4 PO/OH) |
