9DQA

Crystal structure of bovine RPE65 in complex with EYE-002

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	175.810, 175.810, 86.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.906 - 2.100
Rwork	0.180
R-free	0.20890
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.914
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.100	2.100
Rmerge	0.153	2.025
Number of reflections	87750	12591
<I/σ(I)>	8.5	0.8
Completeness [%]	98.8
Redundancy	10
CC(1/2)	0.997	0.298

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	281	100 mM Tris-HCl pH 8.5, 30% PEG 200, 200 mM ammonium phosphate