9DQ1
Crystal structure of HrmJ from Streptomyces sp. CFMR 7 (HrmJ-ssc) complexed with manganese (II), 2-oxoglutarate and 6-nitronorleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-14 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.342, 74.971, 87.295 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 17.490 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.046 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.730 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.077 | |
| Number of reflections | 45410 | 3010 |
| <I/σ(I)> | 14.6 | 5.1 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.997 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M NaI; 0.1 M bis-tris propane, pH 6.5; 20% (w/v) PEG 3350 |
