9DQ0
Crystal structure of apo HrmJ from Streptomyces sp. CFMR 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.496, 75.033, 86.568 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 17.710 - 2.000 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.027 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.710 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.154 | |
| Number of reflections | 38520 | 2860 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.988 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M magnesium acetate; 0.1 M sodium cacodylate; pH 6.5; 20% (w/v) PEG 8000 |
