9DPU
BMP-9 G389S Dimer in Acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 71.589, 71.589, 146.719 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.660 - 2.100 |
| R-factor | 0.1933 |
| Rwork | 0.192 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.029 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.660 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.156 | 1.444 |
| Rmeas | 0.162 | 1.498 |
| Rpim | 0.043 | 0.399 |
| Number of reflections | 11543 | 929 |
| <I/σ(I)> | 13.9 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.6 | 26.9 |
| CC(1/2) | 0.997 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 1M NaCl, 0.1M Acetic Acid pH 4, 27% Glycerol |
