9DPN
BMP-9 Wild-Type Dimer Without Radiation Damage in Neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 71.203, 71.203, 146.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.500 - 2.240 |
| R-factor | 0.1958 |
| Rwork | 0.195 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.678 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 2.310 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.157 | 0.841 |
| Rmeas | 0.199 | 1.078 |
| Rpim | 0.120 | 0.663 |
| Number of reflections | 8929 | 800 |
| <I/σ(I)> | 4.3 | 1.6 |
| Completeness [%] | 95.9 | 96.7 |
| Redundancy | 3.2 | 3.3 |
| CC(1/2) | 0.970 | 0.471 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 1M NaCl, 17% Glycerol, 0.1 M HEPEs pH 7.5 |
