9DP5
Crystal Structure of Hydra Adenosine Deaminase Acting on RNA (ADAR) Deaminase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-11 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.400, 49.440, 83.040 |
| Unit cell angles | 83.11, 80.17, 71.06 |
Refinement procedure
| Resolution | 39.376 - 1.998 |
| Rwork | 0.211 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.050 |
| High resolution limit [Å] | 1.998 | 2.000 |
| Rmerge | 0.094 | 1.123 |
| Rmeas | 0.111 | 1.323 |
| Number of reflections | 47063 | 3516 |
| <I/σ(I)> | 7.79 | 1.23 |
| Completeness [%] | 95.2 | 95.1 |
| Redundancy | 3.52 | 3.54 |
| CC(1/2) | 0.996 | 0.737 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2 M NaCl, 0.1 M Bis-Tris pH 6.5, 25% PEG3350 |
