9DOZ
Myocilin OLF oligomers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-06-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.268, 50.895, 50.490 |
| Unit cell angles | 90.00, 96.68, 90.00 |
Refinement procedure
| Resolution | 37.260 - 1.454 |
| R-factor | 0.1686 |
| Rwork | 0.167 |
| R-free | 0.19640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.260 | 1.506 |
| High resolution limit [Å] | 1.454 | 1.454 |
| Rmerge | 0.148 | 0.724 |
| Rmeas | 0.163 | 0.812 |
| Rpim | 0.066 | 0.362 |
| Number of reflections | 42816 | 3909 |
| <I/σ(I)> | 13.49 | 2.83 |
| Completeness [%] | 97.7 | 89.54 |
| Redundancy | 5.8 | 4.7 |
| CC(1/2) | 0.993 | 0.715 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 10% PEG8000, 0.1M magnesium chloride |
