9DNL

Apo crystal structure of RamR variant R2.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	94.680, 95.220, 130.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.080 - 4.010
R-factor	0.257
Rwork	0.253
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.697
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc3_3805)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.920	3.990
High resolution limit [Å]	3.890	3.890
Rmerge	0.547	3.654
Rmeas	0.597	3.977
Rpim	0.236	1.554
Total number of observations	69577	5160
Number of reflections	11071	804
<I/σ(I)>	3.2	0.7
Completeness [%]	99.9
Redundancy	6.3	6.4
CC(1/2)	0.975	0.264

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M TRIS pH 8.5, 15% PEG 20000