9DNC

Apo crystal structure of RamR variant D2.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 2
Unit cell lengths	90.820, 150.400, 39.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.890 - 1.900
R-factor	0.1977
Rwork	0.197
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.223
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc3_3805)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.890	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.069	1.226
Rmeas	0.075	1.332
Rpim	0.030	0.516
Total number of observations	280002	21038
Number of reflections	43806	3183
<I/σ(I)>	13.2	1.5
Completeness [%]	99.8
Redundancy	6.4	6.6
CC(1/2)	0.998	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Sodium Acetate pH 4.6, 2.0M NaCl