9DNA
CRYSTAL STRUCTURE ANALYSIS OF AN A-DNA FRAGMENT AT 1.8 ANGSTROMS RESOLUTION. D(GCCCGGGC)
Experimental procedure
| Source type | SEALED TUBE |
| Detector technology | DIFFRACTOMETER |
| Detector | STOE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 43.250, 43.250, 24.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.171 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.033 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.027 * |
| Total number of observations | 2599 * |
| Number of reflections | 1553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 * | MICRODIALYSIS |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD OR ISOPROPANOL | ||
| 3 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD OR ISOPROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA | 10 (mg/ml) | ||
| 2 | 1 | Tris-HCl | 10 (mM) | can be replaced with 20mM Na-cacodylate | |
| 4 | 1 | 2 | MPD | can be replaced with isopropanol |
