9DN4
Crystal structure of a SARS-CoV-2 20-mer RNA in complex with FAB BL3-6S97N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-09 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.726, 68.025, 118.615 |
| Unit cell angles | 90.00, 100.16, 90.00 |
Refinement procedure
| Resolution | 47.410 - 1.900 |
| R-factor | 0.1781 |
| Rwork | 0.176 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5177: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.410 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.152 | 1.089 |
| Rmeas | 0.171 | 1.224 |
| Rpim | 0.077 | 0.550 |
| Total number of observations | 364335 | 21115 |
| Number of reflections | 79997 | 4503 |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.996 | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 2M ammonium sulfate, 100 mM Tris pH 8.5, Cryo: 80% crystallization solution and 20% (v/v) glycerol |
