9DMX

Crystal structure of shark UrIg2 V-C1-C2-C3 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	80.952, 97.384, 176.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 2.810
R-factor	0.2503
Rwork	0.249
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.591
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.850
High resolution limit [Å]	2.810	2.810
Rmerge	0.117	2.140
Rmeas	0.124	2.320
Rpim	0.040	0.861
Number of reflections	34210	1655
<I/σ(I)>	17.7	0.8
Completeness [%]	99.4	99.6
Redundancy	9.3	6.9
CC(1/2)	0.998	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2M magnesium formate, 20% PEG3350, 0.1M MES