9DLT

STRUCTURE OF SERINE HYDROXYMETHYLTRANSFERASE 5 FROM GLYCINE MAX CULTIVAR ESSEX COMPLEXED WITH PLP-GLYCINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2022-10-26
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	174.830, 174.830, 184.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.670 - 1.900
R-factor	0.2007
Rwork	0.199
R-free	0.23050
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.670	1.750
High resolution limit [Å]	1.720	1.720
Number of reflections	341703	16864
<I/σ(I)>	10.7
Completeness [%]	99.9
Redundancy	5
CC(1/2)	0.999	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.22 M TRIMETHYLAMINE N-OXIDE, 0.1 M TRIS (PH 8.5), 20% (W/V) PEG MME 2000