9DKI
Crystal structure of the TIR domain C117H mutant from human TRAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 87.021, 87.021, 87.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.530 - 3.000 |
| R-factor | 0.2335 |
| Rwork | 0.232 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.652 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.530 | 3.108 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.061 | 1.725 |
| Rmeas | 0.062 | 1.750 |
| Rpim | 0.010 | 0.290 |
| Number of reflections | 4567 | 433 |
| <I/σ(I)> | 42.35 | 2.44 |
| Completeness [%] | 99.7 | 98.18 |
| Redundancy | 38.7 | |
| CC(1/2) | 1.000 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Li2SO4, 0.1 M Bis-Tris (pH 6.5) and 16% w/v PEG 3350 |
