9DJX

Ternary complex structure of Cereblon-DDB1 bound to WIZ(ZF7) and the molecular glue WIZ-6

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	109.114, 145.029, 191.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.880 - 3.350
R-factor	0.2057
Rwork	0.202
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.048
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.480	3.480
High resolution limit [Å]	3.350	3.350
Rmerge	0.283	4.452
Rmeas	0.289	4.586
Rpim	0.059	1.077
Total number of observations	1025053	81974
Number of reflections	44026	4613
<I/σ(I)>	9.8	0.8
Completeness [%]	98.7
Redundancy	23.3	17.8
CC(1/2)	0.998	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.2 M Sodium Sulfate, 0.1M BIS-TRIS Propane pH 7.5, 20% PEG3350 (Qiagen PACT G8)