9DIN

Structure of ClpC1 N-terminal Domain complexed with semi-synthetic Rufomycin analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12713
Spacegroup name	P 21 21 21
Unit cell lengths	33.960, 58.160, 60.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.370 - 1.640
R-factor	0.1814
Rwork	0.180
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.524
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.010	1.680
High resolution limit [Å]	1.640	1.650
Rmerge	0.071
Rmeas	0.109	0.539
Rpim	0.044	0.205
Number of reflections	15221	514
<I/σ(I)>	25.9	2.2
Completeness [%]	94.2	67.8
Redundancy	5.8	5.1
CC(1/2)	0.997	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.2 M Sodium acetate, 0.1 M TRIS pH 8.5, 16% PEG 4000