9DIB

Rous sarcoma virus frameshifting pseudoknot RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-19
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	43.763, 68.246, 209.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.820 - 2.680
R-factor	0.2113
Rwork	0.206
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.820	2.776
High resolution limit [Å]	2.680	2.680
Rmerge	0.093	1.470
Number of reflections	18350	1815
<I/σ(I)>	22.5	1.62
Completeness [%]	99.4
Redundancy	12.9
CC(1/2)	1.000	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	20% w/v PEG3350, 0.25 M sodium chloride, 0.1 M Tris-HCl, pH 8.5