9DI4
RMI1-RMI2 bound to cyclic peptide L4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 136.298, 136.298, 88.608 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.169 - 2.696 |
| Rwork | 0.169 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.169 | 2.830 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 13881 | 1742 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 99.6 | |
| Redundancy | 38.8 | |
| CC(1/2) | 0.999 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 0.01M zinc chloride, 0.1M sodium acetate pH 5.0, 20% w/v PEG 6000 |
