9DHK

RMI1-RMI2 bound to cyclic peptide L3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	189.150, 50.622, 181.221
Unit cell angles	90.00, 118.05, 90.00

Refinement procedure

Resolution	48.272 - 2.350
Rwork	0.203
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.941
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.272	2.410
High resolution limit [Å]	2.350	2.350
Number of reflections	63378	4427
<I/σ(I)>	5.6	1.1
Completeness [%]	99.2
Redundancy	3.5
CC(1/2)	0.983	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.8 M sodium phosphate monobasic monohydrate / potassium phosphate dibasic pH 5.0