9DHF

The Retinoblastoma Protein with Mutation E554K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	250.356, 250.356, 35.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.310 - 2.260
R-factor	0.2293
Rwork	0.227
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.409
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.310	2.330
High resolution limit [Å]	2.260	2.260
Number of reflections	38644	3613
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	0.997	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	18% PEG8K, 0.1M sodium citrate, 0.1M succinate pH 5.5, 1M lithium chloride