9DGM

T-junction triangle 8-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-08-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	167.490, 167.490, 78.099
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.380 - 6.630
R-factor	0.179
Rwork	0.173
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.277
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.980
High resolution limit [Å]	6.630	6.630
Rpim	0.053
Number of reflections	1462	135
<I/σ(I)>	14.7
Completeness [%]	96.3
Redundancy	6.6
CC(1/2)		0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	DNA strands mixed with TAE/Mg2+ and 0.001 M Magnesium chloride hexahydrate, 0.005 M Tris hydrochloride, pH=7.5, 0.16 M Ammonium sulfate, 0.23 M sucrose and crystallized against (NH4)2SO4