9DG0

T-junction triangle 7-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-08-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	167.218, 167.218, 80.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.320 - 6.530
R-factor	0.1715
Rwork	0.166
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.537
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.630
High resolution limit [Å]	6.400	6.400
Rpim	0.041
Number of reflections	1703	173
<I/σ(I)>	13.5
Completeness [%]	99.3
Redundancy	6.6
CC(1/2)		0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	DNA strands mixed with TAE/Mg2+ and 0.001 M Magnesium chloride hexahydrate, 0.005 M Tris hydrochloride, pH=7.5, 0.16 M Ammonium sulfate, 0.23 M sucrose and crystallized against (NH4)2SO4