9DF1

Q108K:K40L:T51V:T53C:R58Y:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V and irradiated with UV light at pH 7.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127
Spacegroup name	P 1
Unit cell lengths	31.077, 36.177, 64.022
Unit cell angles	86.30, 86.32, 65.23

Refinement procedure

Resolution	31.920 - 1.524
Rwork	0.209
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.920	1.579
High resolution limit [Å]	1.524	1.524
Rmeas	0.056
Rpim	0.040	0.418
Number of reflections	36198	3166
<I/σ(I)>	25.5
Completeness [%]	93.9
Redundancy	2
CC(1/2)		0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, sodium acetate, pH 4.0-4.8