9DEY
Human norovirus GII.4 Sydney protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2023-12-02 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 1.000074 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.210, 158.900, 93.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.540 - 2.400 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.594 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5156) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.540 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.179 | |
| Rmeas | 0.208 | |
| Rpim | 0.102 | |
| Number of reflections | 31740 | 3522 |
| <I/σ(I)> | 4.53 | 2.02 |
| Completeness [%] | 92.9 | 65.72 |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.797 | 0.477 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 1-8% Tacsimate pH 5.0, 4-16% PEG 3,350 |
