9DEP
USP7 in complex with macrocycle MC09
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 244.997, 68.848, 76.052 |
| Unit cell angles | 90.00, 105.21, 90.00 |
Refinement procedure
| Resolution | 73.390 - 2.570 |
| R-factor | 0.2488 |
| Rwork | 0.247 |
| R-free | 0.28410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.579 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.390 | 2.664 |
| High resolution limit [Å] | 2.570 | 2.572 |
| Rmerge | 0.048 | 0.743 |
| Rmeas | 0.058 | 0.869 |
| Rpim | 0.040 | 0.610 |
| Number of reflections | 27109 | 1355 |
| <I/σ(I)> | 9.7 | 1.4 |
| Completeness [%] | 69.2 | 5.32 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% w/v PEG 1500 and 0.1 M MMT (malic acid, MES, Tris) buffer pH 7 |
