9DEB
Crystal Structure of D3-threaded
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-05 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.038, 119.443, 116.126 |
| Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
| Resolution | 46.540 - 2.540 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.26240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.495 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.540 | 2.600 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.167 | 2.056 |
| Rpim | 0.074 | 0.900 |
| Number of reflections | 67007 | 5350 |
| <I/σ(I)> | 9 | 1 |
| Completeness [%] | 99.2 | 99.05 |
| Redundancy | 3.4 | 2 |
| CC(1/2) | 0.998 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.4 M Sodium malonate dibasic monohydrate pH 7.0 |
