9DE1

Q108K:K40L:T51V:T53C:R58Y:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V in the dark at pH 6.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.121
Spacegroup name	P 1
Unit cell lengths	31.032, 35.790, 63.770
Unit cell angles	85.90, 85.87, 65.29

Refinement procedure

Resolution	32.480 - 1.400
R-factor	0.1952
Rwork	0.195
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.156
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.480	1.450
High resolution limit [Å]	1.400	1.400
Rmeas	0.096	0.452
Rpim	0.053	0.053
Number of reflections	46852	4549
<I/σ(I)>	32.23
Completeness [%]	95.6
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	25% PEG4000, 100 mM ammonium acetate, 100 mM sodium acetate, pH 4.0-4.8