9DDD
SufS-SufU complex from Mycobacterium Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.799, 73.616, 76.349 |
| Unit cell angles | 91.03, 100.22, 110.02 |
Refinement procedure
| Resolution | 48.680 - 2.000 |
| R-factor | 0.18058 |
| Rwork | 0.179 |
| R-free | 0.20272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 88203 | 4246 |
| <I/σ(I)> | 10.8 | 2.5 |
| Completeness [%] | 98.0 | 91.9 |
| Redundancy | 7.3 | 7 |
| CC(1/2) | 0.998 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Morpheus 0.1M Carboxylic acids Morpheus Buffer System 1 PH6.5 Morpheus Precipitant mix 1 |
