9DCR
Structure of the TelA-associated type VII secretion system chaperone SIR_0168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-21 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.760, 63.490, 111.990 |
| Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
| Resolution | 39.880 - 1.850 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.564 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.240 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.041 | 0.414 |
| Rmeas | 0.043 | 0.449 |
| Rpim | 0.015 | 0.168 |
| Number of reflections | 36953 | 1341 |
| <I/σ(I)> | 24.5 | 4.5 |
| Completeness [%] | 95.5 | 69.7 |
| Redundancy | 8.3 | |
| CC(1/2) | 0.999 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 200mM Sodium Thiocyanate pH 6.9, 20% PEG 3350 |
