9DCG
Crystal Structure of the Thiol:Disulfide Interchange Protein DsbC from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-14 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.499, 43.744, 47.851 |
| Unit cell angles | 64.07, 80.79, 79.86 |
Refinement procedure
| Resolution | 37.870 - 2.090 |
| R-factor | 0.2167 |
| Rwork | 0.214 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.402 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.168 | 0.168 |
| Rmeas | 0.211 | 0.211 |
| Rpim | 0.125 | 0.125 |
| Number of reflections | 14253 | 567 |
| <I/σ(I)> | 14.21 | 4.08 |
| Completeness [%] | 90.7 | 74.6 |
| Redundancy | 2.4 | 2 |
| CC(1/2) | 0.986 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6 M Lithium sulfate, 150 mM Cacodylate pH 6.5, 1.0 % v/v 1,2 propanediol |
