9DBF
Zn-Bound Structure of a Single-Chain Tet4 Variant with an L42W Mutation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.265100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.060, 46.110, 46.160 |
| Unit cell angles | 104.46, 104.39, 104.33 |
Refinement procedure
| Resolution | 42.180 - 3.200 |
| R-factor | 0.2256 |
| Rwork | 0.219 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.363 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.180 | 3.315 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.081 | 0.702 |
| Rmeas | 0.096 | 0.827 |
| Rpim | 0.051 | 0.432 |
| Number of reflections | 10458 | 1605 |
| <I/σ(I)> | 9.79 | 1.93 |
| Completeness [%] | 97.1 | 96.4 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.997 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 14% PEG1500, 200 mM MgCl2, 100 mM Bis-Tris (pH 6.5) |
