9DBD

Zn-Bound Structure of a Single-Chain Tet4 Variant with an H38E Mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977410
Spacegroup name	P 1
Unit cell lengths	46.040, 46.040, 46.100
Unit cell angles	104.25, 104.34, 104.17

Refinement procedure

Resolution	42.220 - 2.600
R-factor	0.2449
Rwork	0.238
R-free	0.31110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.220	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.052	0.529
Rmeas	0.063	0.648
Rpim	0.034	0.366
Number of reflections	18980	2992
<I/σ(I)>	14.14	2.19
Completeness [%]	95.1	94.64
Redundancy	3.2	3
CC(1/2)	0.999	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	20% PEG 1500, 200 mM MgCl2, 100 mM Bis-Tris (pH 6.5)