9DB5

A DARPin fused to the 1TEL crystallization chaperone via a proline-alanine linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979460
Spacegroup name	P 65
Unit cell lengths	97.730, 97.730, 45.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.870 - 1.570
Rwork	0.167
R-free	0.19630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.052
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	1.626
High resolution limit [Å]	1.570	1.570
Rmerge	0.216	4.199
Rmeas	0.225	4.353
Rpim	0.060	1.140
Number of reflections	33052	3468
<I/σ(I)>	12.34	1.38
Completeness [%]	94.1	99.54
Redundancy	13.7	14.3
CC(1/2)	0.996	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	294	4.0 M Ammonium acetate, 0.1 M Sodium acetate trihydrate