9DAY

Crystal Structure of the Transcriptional Regulator HcpR from Porphyromonas Gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-22
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	106.281, 106.281, 116.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.570 - 2.300
R-factor	0.2171
Rwork	0.215
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.041
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.570	2.370
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	0.537
Rmeas	0.059	0.593
Rpim	0.025	0.247
Number of reflections	29963	2938
<I/σ(I)>	24.6	3
Completeness [%]	99.0	100
Redundancy	5.4	5.4
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1M Na Acetate, pH 4.6, 1.8M Na Formate