9D9V
X-Ray structure of ALX4 homeodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2022-12-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 95.780, 95.780, 95.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.860 - 2.390 |
| R-factor | 0.2177 |
| Rwork | 0.214 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.016 |
| Data reduction software | MOSFLM |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_4788: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.730 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.119 | 2.810 |
| Rmeas | 0.122 | 2.884 |
| Rpim | 0.026 | 0.646 |
| Total number of observations | 130519 | 12377 |
| Number of reflections | 5957 | 626 |
| <I/σ(I)> | 14.1 | 1.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 21.9 | 19.8 |
| CC(1/2) | 0.999 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1M MgCl2, 0.1M KCl, 0.1M PIPES pH=7.0, 20% PEG Smear Medium |
