9D9P

Crystal structure of CVB3 replication-linked RNA in complex with 3C protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	106.406, 95.251, 113.637
Unit cell angles	90.00, 98.63, 90.00

Refinement procedure

Resolution	37.450 - 2.690
R-factor	0.2003
Rwork	0.198
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.149
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.450	2.740
High resolution limit [Å]	2.690	2.690
Number of reflections	59666	5248
<I/σ(I)>	19.2
Completeness [%]	99.8
Redundancy	3.4
CC(1/2)	0.997	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291.15	Glycerol, Bis-Tris, Ammonium Sulfate