9D9O

Crystal structure of CVB3 replication-linked RNA stem-loop D in complex with 3C protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	52.300, 78.869, 106.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.130 - 2.410
R-factor	0.1987
Rwork	0.194
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.111
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.870	2.450
High resolution limit [Å]	2.410	2.410
Rmerge	0.091	0.681
Number of reflections	32615	5449
<I/σ(I)>	13.8	1.2
Completeness [%]	100.0	96.5
Redundancy	6.7	6.6
CC(1/2)	0.997	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291.15	DL-Malic Acid, PEG 3350