9D96
KIR3DL1 - HLA-B38-YHL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95364 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.346, 60.588, 63.288 |
| Unit cell angles | 95.06, 95.61, 106.37 |
Refinement procedure
| Resolution | 41.970 - 2.400 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.515 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.970 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 25635 | 2545 |
| <I/σ(I)> | 8.13 | 1.69 |
| Completeness [%] | 97.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.978 | 0.597 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 15% PEG 3350, 2% Tacsimate, pH 5.0, and 0.1 M trisodium citrate, pH 5.6 |
