9D95
TRAV35/TRBV20 TCR - HLA-B38 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.789, 124.060, 332.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.800 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rpim | 0.064 | 0.437 |
| Number of reflections | 96562 | 9725 |
| <I/σ(I)> | 9.71 | 2.12 |
| Completeness [%] | 91.5 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.994 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Bis-Tris, pH6.5, 22%PEG3350, 0.2M (NH4)2SO4 |
