9D8N

Q108K:K40L:T51V:T53C:R58W:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V and irradiated with UV light at pH 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	31.100, 35.997, 64.013
Unit cell angles	86.11, 86.50, 65.10

Refinement procedure

Resolution	28.510 - 1.301
Rwork	0.203
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.066
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.510	1.347
High resolution limit [Å]	1.301	1.301
Number of reflections	58664	5741
<I/σ(I)>	15.9
Completeness [%]	95.2
Redundancy	5.3
CC(1/2)	0.879	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, sodium acetate, pH 4.0-4.8