9D8M

Q108K:K40L:T51V:T53C:R58W:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V fluorophore in the dark state at pH 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	31.171, 35.859, 64.046
Unit cell angles	85.66, 86.04, 65.17

Refinement procedure

Resolution	28.270 - 1.201
Rwork	0.193
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.876
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.270	1.244
High resolution limit [Å]	1.201	1.201
Number of reflections	74135	7114
<I/σ(I)>	21
Completeness [%]	94.8
Redundancy	5.9
CC(1/2)	0.878	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, sodium acetate, pH 4.0-4.8