9D5X
Structure of G2L4 RT Apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-14 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.836, 73.227, 82.861 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 48.750 - 2.610 |
| R-factor | 0.2184 |
| Rwork | 0.217 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.430 |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 2.720 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.055 | 0.670 |
| Rmeas | 0.060 | 0.728 |
| Rpim | 0.023 | 0.282 |
| Total number of observations | 183499 | 21310 |
| Number of reflections | 27113 | 3290 |
| <I/σ(I)> | 20.8 | 2.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.999 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295.15 | 0.1M Bis-Tris, 0.2M Ammonium acetate, 25% PEG3350 |
