9D5W
Crystal Structure of the Substrate Binding Domain Protein of the ABC Transporter PBP2_YxeM from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-29 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.044, 105.816, 118.124 |
| Unit cell angles | 90.00, 119.92, 90.00 |
Refinement procedure
| Resolution | 59.110 - 2.300 |
| Rwork | 0.194 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.261 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.110 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.114 | 0.469 |
| Rmeas | 0.141 | 0.578 |
| Rpim | 0.082 | 0.333 |
| Number of reflections | 107373 | 4978 |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 95.8 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.876 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.6 M Lithium sulfate, 150 mM Cacodylate pH 6.5, 1.0 % v/v 1,2 propanediol |
