9D5L

The C-terminal domain of Thiopseudomonas alkaliphila Tn7 TnsE bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-29
Detector	RAYONIX MX300HE
Wavelength(s)	1.0332
Spacegroup name	P 61 2 2
Unit cell lengths	63.770, 63.770, 220.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.380 - 2.800
R-factor	0.2403
Rwork	0.237
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	7169	702
<I/σ(I)>	9.03
Completeness [%]	99.6
Redundancy	38.4
CC(1/2)	0.995	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Sodium Iodide, Bis-Tris propane, PEG 3350